Overview
Beverly Hills Plastic Surgery Group is thrilled to introduce you to Proactive Longevity—a new extension of the Beverly Hills Plastic Surgery Group, designed to help you manage the trajectory of aging from the inside out.
While our surgical team has long focused on refining external beauty, Proactive Longevity complements that vision by offering advanced, science-based regenerative therapies that target cellular health, immune strength, and total-body rejuvenation.
At the heart of our approach is 3D Anti-Aging Science™, a proprietary protocol designed to:
- Detoxify the body through Therapeutic Plasma Exchange (TPE)
- Defend the immune system with NK cell therapy
- Deliver lasting vitality through precision stem cell applications
Full Transcript
00:33
Dafne Canales
Hello and welcome everyone and thank you for joining us for beyond the Surface, a new era in longevity and wellness. Before we dive in, we’d love for you to tell us your name and where you’re joining us from in the chat. Down at the bottom. It’s always incredible to see how far this community reaches. And one important reminder, please stay until the end of the webinar. All attendees will be automatically entered for a chance to win a complimentary $3,500 microneedling exosome facial. The winner must be present during the live drawing at the end to claim the prize, so don’t go anywhere. Now, let’s begin. We’re thrilled to welcome you on behalf of Beverly Hills Plastic Surgery Group, a practice long dedicated to enhancing outer beauty through surgical excellence. Today, we’re proud to introduce an exciting evolution of that mission. Proactive Longevity.
01:23
Dafne Canales
Proactive Longevity is a new division of our practice is built to help you manage the trajectory of aging not just on the surface, but deep within. While esthetic refinement remains a core part of what we do, we believe that true vitality begins at the cellular level. That’s why we’ve developed a science based approach called 3D Anti Aging Science, a proprietary protocol designed to detoxify the body through therapeutic plasma exchange, defend immune strength with natural killer cell therapy, and deliver lasting energy and resilience using precision stem cell applications. And you’ll find out more about that shortly. This powerful combination of regenerative therapies is designed not only to help you look your best, but to feel your best for years to come. Now it’s my pleasure to introduce the visionary behind this movement, Dr. John Layke. Dr.
02:18
Dafne Canales
John Layke is the Chief Medical Officer and co founder of Proactive Longevity.
02:23
Dr. John Layke
He’s.
02:23
Dafne Canales
He is board certified in both general surgery and plastic and reconstructive surgery and also serves as the co founder of Beverly Hills Plastic Surgery Group and the internationally recognized skincare line, Beverly Hills, Maryland. Dr. Layke has authored numerous journal articles in both national and international publications, contributed to book chapters on skin and aging, and brings over a decade of experience in regenerative medicine. His current research focuses on cutting edge advancements in stem cells, exosomes and anti aging science. Please join me in welcoming Dr. John Layke.
03:00
Dr. John Layke
Hey guys, thanks for joining me today and I wanted to thank you really for spending your time. What I’m really hoping to do here over the course of the next 30 to 45 minutes is kind of take you on a journey on our thought process behind reversing or Slowing down the signs of aging. And at proactive longevity, we think that aging is not just about adding years to your life, it’s more about adding life to your years. And I’ll kind of go into more detail on the managing the trajectory of aging in 3D. The detox defend and deliver vitality. We’ll go into that and kind of break it down as to why we feel that our process has served our clients well and why it will continue in the future.
03:45
Dr. John Layke
We’ll talk about, you know, advanced regenerative therapies like stem cell treatments and other protocols, and designing kind of a personalized roadmap for each and every patient. And so we can help you live better, not just longer. And to start, I’d like to show you a high level video which details everything we’re about to dive into. How long of a life do you picture for yourself? More than just saying a number. How do you want to live as you age? Before you answer, did you know that you’re able to significantly extend the quality years of your life? Do you remember sleeping through the night without getting up once? Do you remember never having to strain to read the fine print on a dimly lit menu? Or never limiting your body movements due to wear and tear? Aging is now a treatable condition.
04:43
Dr. John Layke
We at proactive longevity are going to help you reprogram your cells to a younger state, in essence biologically reversing your age. Our advanced therapeutics utilize cutting edge longevity science, which includes stem cells, exosomes, nutraceuticals and peptides. Stem cells seem to have established a before and after in the timeline of what is possible in regenerative medicine. Let me take you on a journey back to the beginning, then show you where we are today and how we can positively impact the trajectory of aging. And the great news is I’m not alone. You know, at proctive longevity, we have a team of physicians, some who you may know, that will work together with you to create the perfect regenerative plan. Plan.
05:33
Dr. John Layke
So now you’d have to live under a rock to not have heard about the longevity and wellness space and possibly stem cells or exosomes or the term regenerative medicine. And hopefully what I’ll do is kind of dispel some of the myths and briefly introduce some of therapies that are currently available. And maybe you’ve, you’re confused, you’ve heard enough or there’s too much information out there, you’re trying to sort through the noise. And so the real question is, you know, Are stem cells really the new next evolution in medicine? Is this really the duct tape or the Windex? We feel it is. So who’s a candidate? You’ve read a lot about stem cells online, but again, sorting through the truth can be kind of challenging. And celebrities may endorse treatments, but no one really knows what’s going into their bodies. Right.
06:22
Dr. John Layke
And we’d like to describe these as not just trendy procedures, but ones that will significantly impact our trajectory of aging. Or if you’re ready to see aging as a treatable condition, you want to be around, active, and engaged for your loved ones. So really, the question that we asked, you know, it’s not, how long of a life do you picture for yourself? Because no one wants to live to be 150 if they can’t move, it’s how well do you want to live? How do you want to live as you age? That’s why we’re meeting here today. We already have multiple examples of centenarians that maintain healthy and active lifestyles. So in 2021, there were almost 90,000 centenarians in the United States. Now, the population here is supposed to quadruple over the next 30 years. This is across the world.
07:16
Dr. John Layke
As of 2020, it’s almost 600,000 centenarians. And with centenarians on the rise, if we don’t do something to live better, the health care system everywhere will be upended. There’s something called longevity escape velocity. Now, this is the concept that if our grandparents lived until our 70s, our parents live until our 80s. The next common thought, logically, would be that we live until our 90s. The problem with this philosophy is that AI has altered medicine indefinitely. And we’ll put it as an example, when I worked at a lab in medical school, Iran protein gels. Some of these studies would take an entire year to come up. With these samples, these same studies are being performed in seconds.
08:04
Dr. John Layke
So beyond 2050, if artificial intelligence, quantum computing, and nanotechnology accelerate biomedical research, a full cure for aging, which includes maybe biological age that can be reset indefinitely, could def. Could be possible. So what stands in our way? These are the four biggest killers. We call them the four Horsemen. This is cardiovascular disease, cancer, metabolic syndrome, and neurodegenerative disorders. What is it about aging that makes your heart go bad or be able to create cancer, affect your pancreas and your brain? The real question is, why do we feel that aging is a treatable condition? What do all diseases have in common? And this is where proactive longevity introduces our concept of managing the trajectory of aging in 3D. And we are using science and data backed solutions. So a paper was published years ago that elucidated the nine hallmarks of aging.
09:10
Dr. John Layke
And we’ve categorized these into three different categories. Now, the first one being chronic inflammation. This is where you have altered intracellular communication. This is a change in how cells actually communicate with each other. Or maybe mitochondrial dysfunction. For those of you take NAD or you’ve had NAD drips. The idea is that these are the energy powerhouses of the cells that form ATP and these processes slowly break down. If a cell’s not making energy, it can’t do what it’s supposed to do. And deregulated nutrient sensing. These are a breakdown of the systems that control metabolism and energy production. And so we consider this all chronic inflammation or inflammaging. The second category is senescent cells. These are aging or what are called zombie cells. And when we look at the hallmarks of aging that are kind of grouped under this category, it’s more like epigenetic alterations.
10:08
Dr. John Layke
It’s the expression of your genes. These are chemical changes to the DNA that affect gene expression without changing or altering the actual DNA sequence. And this can be passed down from generation to generation and affect your risk of disease and then loss of proteostasis. This occurs when the delicate balance is disrupted, leading to accumulation of misfolded, aggregated or damaged proteins. And so it leads to essentially garbage cells. And the last category is depleted stem cell reservoir. So when we look at stem cell exhaustion, you’re born with a number of cells and there is a significant decline in not only the number, but the function of these particular stem cells. So we have reviews, data to combat each one of the three categories. And the first one is detox. We talk about detoxing the inflammation. The second is to defend your immunity.
11:12
Dr. John Layke
And the third is to deliver vitality. So how do we do this? Step one, detox. Why combat inflammation? Inflammation is one of the root causes of aging. And this is where toxins and immune complexes that build up and circulate through your system, they lead to chronic inflammation and ultimately it affects heart health, causes joint pain. And over time, this chronic low grade inflammation can damage cells, tissues organs. And what this does, it accelerates age related changes and increases the risk of many diseases that are associated with aging. They deplete immune function, obstructs metabolic health, and even impedes digestive health. We’ve all heard about the gut brain axis and all of you have heard that know how important that is. We already know the basics. Exercise regularly, reduce stress, whether it’s through meditation, unwind, whatever it is, and healthy eating.
12:12
Dr. John Layke
But really, there are only so many blueberries that you can eat. And we need to do something else as the daily damage occurs, whether you follow these rules or not. So what’s next? We look at altering or reversing our own aging process. Heterochronic parabiosis was something that was a study that was introduced early in the 1950s. It was really successfully published in 1972. And this was where a younger mouse was sutured to an older mouse. And the findings were incredible. The older mouse actually lived 30% longer and they had characteristics of looking and acting younger. A recent heterochronic parabiosis study that was recently published in Nature Aging demonstrated that prolonged exposure to the younger mouse ultimately gave the older mouse the younger mouse’s properties even after being divided from the younger mouse. So enter.
13:16
Dr. John Layke
You know, this is where we look at giving someone younger plasma. The original thought process was if we can give someone younger plasma. And this, you know, if any of you have watched, you know, Silicon Valley, you saw that the wealthy executives had their blood boys and they were taking the plasma of younger individuals to feel younger. And I even knew of a 58 year old female who was transfused younger plasma and she started to have her periods again. The problem was the rash that she developed from the immediate infusion. And this is where we ran into some issues and. But here’s the thing with the parabiosis studies. We realized that it wasn’t what the young mouse was actually giving to the older mouse. It was what was actually happening to the older mouse’s plasma. This was dilution. Enter therapeutic plasma exchange.
14:09
Dr. John Layke
And this is a treatment that’s recently popularized for its longevity effects. It’s been around since the early 1900s and it was therapeutically used in 1952 for something that was called hyperviscosity syndrome, which is essentially thickened blood. This is our partner and anesthesiologist, Dr. Joseph Lowenbein. And he’ll kind of go over the tpein here.
14:32
Dr. John Layke
Attractive longevity here in Beverly Hills at our office doing something really cool today, doing therapeutic plasma exchange. What is therapeutic plasma exchange? It’s in essence taking the liquidy portion of your blood which is the plasma, filtering it out. Here we are just doing it through one IV in my body using the machine that we have in our office. And why are we taking the plasma away? Because the plasma has all the old cellular waste Products that contribute to the aging process. And we’re replacing it with albumin. Here the back bag is the plasma, and the albumin containers are in front. And the albumin has great immune properties and is a powerful antioxidant.
15:15
Dr. John Layke
And so we’re doing this for overall wellness, longevity, and really, hopefully trying to avoid the buildup of all the impurities that are happening in your body with the inflammatory processes that are happening and hopefully just warding off everything that we don’t want as we age.
15:33
Dr. John Layke
So when we look again, and Dr. Lombine is holding his entire blood volume of plasma, Right? So plasma makes up 55% of our entire blood volume. So what happens? And you can do this through either one or two IVs, depending on the length of time this takes. And so what happens is the entire circulating blood volume leaves your body heads through one of the most complex machines on the planet, which happens to filter and pull out the plasma again, that contains all of the toxins and immune complexes, BPAs from plastics, heavy metals. And for those of you with autoimmune conditions, this is something that clears those triggering immune complexes. And if you. You take a look, and then what we do is we replace this with fresh, clean albumin.
16:26
Dr. John Layke
So imagine even looking at this particular picture here, we see that you can’t necessarily see through Dr. Lowenbind’s plasma, but what we’re replacing it with is almost crystal clear. And it’s interesting to note when, you know, analogy I often use when the body is kind of like an aging older car, right? And so the tread of the tire goes, the oil gets thick and murky. The windshield, you can’t see through in the gas. You know, you essentially have an empty tank. TPE, or therapeutic plasma exchange, is like the oil change for your body. And this is an example where when we look at normal plasma, which is on the right side compared to someone who is about 40 years old, that’s in the middle.
17:10
Dr. John Layke
And then when you look on the left, we see someone who’s actually has high triglycerides as well as high cholesterol. And so this is an amazing procedure. This is a literal oil change for the body, because we’re filtering out all of the. Essentially the crud that taxes your body and makes your immune system work on overtime. This is one way of slowing down the aging process. But what else can we do? And I want you to take a look. You can see the sediment and cholesterol during a particular patient’s removal. This again, is filtering this from your body. Imagine how much better your body can work. And we are using data backed 3D anti aging science.
17:55
Dr. John Layke
And so we collect pre and post labs looking at inflammatory biomarkers, immune function and screening physiology to actually show you that there is an improvement alongside the physical improvements that you feel. Now we get to step two, which is defend. And we talked about senescent aging or zombie cells. Why do we need to eliminate these zombie cells? So now that we’ve cleared our body of all the toxins and immune complexes that accumulate, how can we change the fact that have we aging or senescent cells, they deplete metabolic health, that causes aging through organ dysfunction and it even promotes tissue damage. Now imagine this is again analogy garden of youth. Over time some of the plants wither, leaving behind these zombie sort of plants that no longer contribute, but still drain resources and cast shadows all over the thriving flora.
18:49
Dr. John Layke
So the gardeners, your body’s repair mechanisms struggle to replace them as the seed bed depletes and renewal slows. So clearing the zombie cells in our body leads to obvious healthier physiology. And again, going back to the car analogy, aging is like a dirty windshield with you. You’re on a road trip. With each splat of a bug, you end up focusing more and more on the windshield itself rather than the road ahead. And along the same lines, the body gets preoccupied cleaning up messes that are created, losing the ability to clearly heal degenerative processes and what the body is supposed to be doing. Enter the windshield wipers. This is known as natural killer cell therapy. And these natural killer cells target a foreign cell, attach to it and release these cytotoxic granules that destroy it.
19:40
Dr. John Layke
Upon activation they can produce cytokines like interferon gamma, which can further activate other immune cells. So imagine not only is it destroying these foreign cells, but it’s activating, ramping up your own immune system. They actively patrol as immune surveillance. And they don’t require any antigen presensitization. They only recognize non self and destroy it. That’s why this can be just a phenomenal treatment, natural killer cell therapy. So you have to imagine what happens is we pull blood. Usually while you’re undergoing therapeutic plasma exchange, we send it to a lab which grows and clonally expands those cells. Then at a future date, those are re injected into your body, destroying tumor cells, viral infected cells. We look at the destroying these zombie cells as well. And this offers your body a chance, your immune system to now focus on what it does best repair slows aging.
20:45
Dr. John Layke
This Treatment enhances metabolic health, it prevents cancer development, and it boosts the effectiveness of all the other therapies. But why the step three? Now we talk about delivering, but why replenish stem cells and exosomes? Right. We are already told that we have a certain number that we have in our body. We’ve reduced inflammation, we’ve cleared your aging or senescent cells. How do we replenish your body? With the tools it needs to heal and regenerate itself. So when we look at a depleted stem cell reservoir, we look at, again, I had alluded to it earlier, it really is stem cell exhaustion. It’s a decline in the number of stem cells and their ability to renew themselves. And so first, what are stem cells? I’m just going to give you.
21:33
Dr. John Layke
Simply put, stem cells are unspecialized cells that can divide and renew themselves over a long time. They can develop into specialized cells such as muscle cells, blood cells, brain cells. And there are really two different types. We call them embryonic and adult. And adult is anything that’s post embryonic. So even umbilical cord is still considered adult stem cells. And really what we’re going to do is just focus on adult stem cells, because the use of embryonic cells is unethical. And, you know, any attempts to try to create embryonic cells, when we do infuse those sometimes can cause tumor growth, things called teratogens. Remember, these stem cells can form any type of tissue. So imagine getting an infusion, and all of a sudden you develop a lump that has hair, teeth, and nails in it. So we’re obviously going to avoid those.
22:26
Dr. John Layke
And then back to that garden youth analogy. So imagine your body as this flourishing garden, where each plant represents a cell that’s vital to the ecosystem’s balance. And so at the heart of the garden lies a bed of magical seeds. And those are the stem cells capable of transforming into any plant the garden needs for renewal and growth. And stem cell replenishment is like restocking the seed bed with fresh, potent seeds. And these new seeds replace the lifeless plants, revitalizing the entire garden. Similarly, replenishing stem cells gives our bodies the tools to repair the damage, combat disease, and maintain health. And keeping the gardener youth vibrant and thriving for years to come. And that’s why it’s extremely important.
23:11
Dr. John Layke
So we know that we’re born with them, we know that we have them, and that they contain the ability to form any cell in the body. And again, to hammer home the problem as we age, the number and efficacy of our own Stem cells reduce drastically. So do stem cells really work? I’ve already alluded to the many studies in regenerative medicine, but don’t just take my word for it. Over the past two decades we’ve used stem cells to treat damaged cardiac tissues, post heart attack, spinal cord injuries, we’ve restored eyesight, we’ve treated cancers with immunotherapy. And There are over 8,400 registered clinical trials currently using stem cells. And yes, they work. So what makes our cells different?
23:56
Dr. John Layke
Why proactive longevity cells other than the fact that we use stem cells from a CGMP certified facilities that are held to the highest standards, Day one in age, genetically tested for abnormalities, non vaccinated, viral and bacteria free. Each batch that’s tested for viability and function. It’s because we offer a specific line of stem cells. Now enter the super power stem cell. These are called muse cells. They were discovered in the early 2000s. They were by a science, Japanese science, named Dr. Mari Dezawa. They were first reported in 2010. Now the interesting part about these cells, these are called muse cells, multilinear, differentiating, stress enduring stem cells. What does that mean? These cells are stress tolerant and can survive in damaged tissue. That’s why they work in dead tissue after a heart attack. That’s why they work in a damaged spinal cord.
24:56
Dr. John Layke
That’s why they can work in knees that have little to no cartilage. They can differentiate into tissue compatible cells without forming teratomas. I talked about this before. This is likely the closest thing to an embryonic cell that can form every type of tissue without forming those tumors that we talked about that can develop hair, teeth, nails and other germ cell layers. The other interesting part about these cells is they actually have receptors that express what’s called sphingosine 1 phosphate. S1P is a protein that’s produced by damaged tissues. So essentially this acts as a homing mechanism that once infused into the body, travels towards that diseased tissue that’s letting off that particular protein. They can repair tissues in the brain, liver, skin, and as I talked about, it’s the closest thing to an embryonic cell.
25:53
Dr. John Layke
So years ago, in the mid-2000s, a particular, a Japanese, another Japanese scientist, it was able to convert an adult stem cell all the way back to its embryonic state. And they had found four, what are called Yamanaka factors. And these are essentially expression like proteins that are markers that allowed the reversal of age. Essentially the problem was we ran into again the teratoma issue. These have three out of the four. These Pluripotency markers. And that’s why without the lack of that fourth one, we find that they form any tissue without developing those tumors. They’re able to self renew so they can continuously replicate themselves. And they also have powerful anti inflammatory, anti fibrotic, which means anti scar and anti apoptotic effects. So they, they avoid destroying cells. Doc. Dr. Jezawa introduced the new supercell when it comes to tissue repair.
27:01
Dr. John Layke
And science has run with it ever since. And they’ve effectively, as we said, treated pain, strokes and all inflammatory conditions with amazing success. Now what are exosomes? If stem cells we consider the Batman of regenerative medicine, then exosomes are definitely the robin. And how can we amplify the effects of stem cells? Enter exosomes. Exosomes carry these cell specific cargo to other cells where they can reprogram the recipient cell. That means they’re carrying the information, these are the messengers and they play a role in many processes including immune response, neuronal communication between nerves and cancer biogenesis. So we can even use these as far as cancer treatments. They communicate the data we need given to the host cells. And this is essentially just a transferring of data, so to speak. And what are the applications of stem cells in exosomes?
27:57
Dr. John Layke
So how do we use these building blocks? And are they synergistic? Can you do one or the other? Well, the answer is yes, they are synergistic. Can you use them separately? Yes, of course. Obviously, using them together they have added benefits. The stem cells carry the youthful information. The exosomes transfer the youth to your cells, essentially. And we can inject the scalp to turn on dormant hair follicles. We can microneedle the face to improve collagen synthesis. And we can even inject joints with or without a gel scaffolding to help repair degenerative joints. We can stave off surgical procedures based off of regenerative capabilities. And when we infuse these cells, we allow these signals to be directed to where the body needs it most. So when we talk about managing the trajectory of your aging in 3D, we detox that’s inflammation with therapeutic plasma exchange.
28:51
Dr. John Layke
We defend this is your immunity with NK or natural killer cell therapy and delivery with a deliver vitality with stem cells. And this is our proprietary solution at proactive longevity. The ways that we feel we can slow down and reverse the causes of aging. Look, with current medical technology and the application of artificial intelligence and science, you’re already likely to live beyond 100. The problem we run into the United States is the fda. And although the climate is changing and RFK tends to be very pro regenerative medicine, we still have code of federal regulations and it’s CFR Title 21 Part 1271, it states that all biologics have to meet three parameters. So biologic is any type of biological material that we are introducing into the body. Here in the United States the three parameters are it has to be performed in the same sitting.
29:55
Dr. John Layke
So that means if we are taking your cells or things out of your body, it can be processed and placed back in the same sitting. This also means that if it comes from a donor, we can’t necessarily do that. The second is minimal manipulation. Sometimes you cannot be cultured, drone or expanded. And so it really limits the ability. Just like the natural killer cell therapy, we have our circulating natural killer cells, but we grow these, expand them and deliver them to the body at least three to four times the concentration. So it allows it to cut through what your normal immune system can. The last is homologous use. That’s the other parameter. Whatever we take the tissue out for, it has to go back in and serve the same purpose.
30:43
Dr. John Layke
And so if you’re removing your own fat cells and we’re, you know, processing fat and allowing it to extract stem cells from the fat, you can reprocess it and put it back into the body as structural building materials. But if we’re trying to do it for anything else, the FDA says we’re running afoul. So here we can’t grow stem cells. You can’t tell if they’re alive because we can’t do serious testing to it. You can’t tell how many there are and you can’t tell if they’re healthy. So that’s why we have to do this outside of the country for now. Seven years later, you know, after flying to Taiwan, China, Europe and Penn, talking to Panama and Costa Rica, we have found some of the latest and greatest.
31:32
Dr. John Layke
We’ve established a cutting edge clinic here at Diamante Los Cabos, which is a Tiger woods golf resort in Cabo San Lucas, Mexico. And here we can, we have official licensing by the FDA associated with Mexico, which is Cofa Priest. We have one of the few true regenerative medicine licenses allowing us to deliver these therapies. And we follow American standards. And so this, we’re following all of the safety standards that we would here in the United States. This is a clinic that has all the license and regulatory accreditations and again it’s run by American standards.
32:17
Dafne Canales
Thank you, Dr. Layke, for the insightful presentation and for sharing the science and vision behind proactive longevity. I love how you said stem cells are the Batman and exosomes are the Robin. I’ll never forget that. At this time, we’d like to move into our Q and A session. We’ve received several excellent questions in advance from attendees and some on the chat, and we’ll start by addressing those. And if you have any additional questions during this time, please feel free to drop them in the chat and we’ll get to them as soon as we can. Also, if you haven’t already, please add your name in the comments so we can enter you into the draw for the complimentary $3,500 micro needling exosome facial. And you must be present at the time of the draw to win. So let’s begin with the first question.
33:08
Dafne Canales
We are going to start with. How often is TPE recommended? A question from Mike L. Amazing.
33:16
Dr. John Layke
So therapeutic plasma exchange, you have to imagine in an ideal world, so we look at a single session because it cleans the blood volume, the entire blood volume. Once in a single session, it removes approximately 66% of all of the toxins in your plasma. The second session, which would be closer to 75%. The third is 85%. The fourth would be 98%. So doing it even once will significantly filter the blood and give your immune system a chance to do what it does best. In an ideal world, as I mentioned earlier, if you did this four times a year, you would get close to 100% of that clearance. But again, even once annually, and it will significantly remove the toxins.
34:08
Dafne Canales
Thank you. Our next question is, what does the research say about TPE’s effectiveness around cancer prevention and what are some of the risks?
34:18
Dr. John Layke
Right. So therapeutic plasma exchange has been used for several blood cancers. And so, you know, the idea behind this, again, the whole idea behind therapeutic plasma change, it’s the body’s oil change. What it’s doing is you are allowing the body to function. You know, again, if you change the oil in your car, it runs smoother. The same thing applies to your body. So if you are, you know, essentially removing all of those toxins and heavy metals and some of the spike proteins from long Covid even, the idea is that your immune system no longer has to focus on those things and all it can do is now repair. Repair damaged tissue.
35:03
Dafne Canales
Thank you. So if someone has autoimmune disease or the immune system is already on overdrive, what is the effect of natural killer cell therapy?
35:13
Dr. John Layke
Sure. So you know, this is where I tie in therapeutic plasma exchange. Again. So TPE is great for some autoimmune conditions. There are some where the immune complex is formed, form so quickly that within a matter of days everything that would be cleared would restore some of the immune. Autoimmune conditions can be very challenging. But you know, as far as those with thyroid issues, Hashimoto’s graves. When we look at, you know, lupus, even when we look at some of these conditions, to even put them at bay for three to six months is very significant. And it gives your body a chance to essentially right itself. Now when you talk about natural killer cell therapy, it really is there to destroy those aging senescent or zombie cells.
36:02
Dr. John Layke
And so it’s really just this, these cells that are taking up some of the resources by the body. And so what happens is the reason why it’s important these cells can trigger your own immune system. So the body sees it as foreign. When the natural killer cell therapy comes in and destroys these cells and it’s cleared now, you have less of immune reactions. And so, you know, there’s data that’s showing that even decreased histamine responses for those who have severe allergies. And so, you know, again, I think these are these three categories that we’ve talked about of aging. Two out of the three can be accomplished just through TPE and natural killer cell therapy alone.
36:48
Dafne Canales
Thank you. We have one of the questions submitted in advance is where do the stem cells come from and how can you ensure that they’re not carrying someone else’s DNA or diseases and you know, having that transferred through.
37:02
Dr. John Layke
Excellent question. So again, we’re following American standards. So you see GMP certified facilities, ISO as far as cleanliness and these are all pre screened. So there are several different types of stem cells allowed at or offered at Proactive Longevity. All of the labs will test and pre screen for any hiv, hep C, prion diseases, bacteria, viruses. And then there’s genetic testing to essentially make sure that we’re, even though we’re not transferring genetic data. The idea is we are trying to maintain as pure of a sample as we can. And these cells themselves usually come from placentas, umbilical cords from young females, otherwise young, healthy donor, adipose or fat derived tissue. And so again, depending on the muse stem cell, the muse comes from particular labs in Europe that follow similar standards and accreditation.
38:09
Dafne Canales
We have another question about muse cells. How are they different from mscs? Can you explain that a little bit further?
38:16
Dr. John Layke
Well, muse cells are MSCs, right. But there are specific types. So MSC is mesochimal stem cell and MUSE is multi lineage differentiating stress enduring mesenchymal stem cell. Right. And so ultimately these particular cells are stressed in a particular environment. Whether it’s a scientific machine called a bioreactor or if we reduce the oxygen tension, these particular cells become the supercells because they are, they stay alive in a stressed environment. The MUSE stem cells obviously, as you can imagine, are harder to ignore extract and therefore sometimes they can be more expensive, but they tend to last longer. And again, the difference is that they contain receptors for SP1, which is the sphingosine 1 phosphate. That’s the receptor that looks for the protein that’s exuded by the body and damaged tissue. And they also contain those embryonic surface markers.
39:15
Dr. John Layke
And it’s the closest thing to an embryonic cell without having to or without the ability to be tumorigenic. Very special cell.
39:26
Dafne Canales
We have another question about the viability of cells. So when, how can you tell that the cells are viable and alive once you’ve received them?
39:36
Dr. John Layke
Right. So as soon as they sell, every single batch is tested for viability. So a certain percent is tested and it’s run through a particular machine. And it’s not just looking at the cell number. This is where many of the clinics get it wrong. It’s not necessarily the number of cells that you’re getting all the time, it’s the quality of the cells. And so we test for the actual viability of the cells and their functional status. Whereas many of the clinics around the world, they offer you 2 to 400 million stem cells and many of those are dead. They pass through a cell sorter and it’s just counting the number, but it’s not actually looking at the viability. So what we do is we test it and then immediately freeze it in liquid nitrogen.
40:30
Dafne Canales
Another question from the chat, where are you sourcing your stem cells from?
40:36
Dr. John Layke
So I kind of alluded to that earlier. So we have multiple different stem cell lines and some are from Mexico. Again, they’re young, healthy donors and we young, healthy placenta and umbilical cord. And so we also have the MUSE stem cells, I said come from both Japan and Europe. And we also have stem cells that come from a lab here in the United States. And so again, what we’re trying to do is we source the highest quality, purest stem cell that has the best functional status.
41:16
Dafne Canales
Another question, do you need all three procedures tpe, NK and stem cells for maximum effectiveness?
41:24
Dr. John Layke
Well, look in an ideal world you would. If there are three essential categories of aging, you’d like to combat each one of them. Is it necessary? No. But you have to start soon somewhere. Even doing a therapeutic plasma exchange again, you are stripping all of the toxins that you’ve been exposed to for the last several decades. And to me I always think that’s a great start. For those who don’t want to undergo a longer procedure, one of the easiest things to do is just refill the tank and that’s where you have a stem cell and exosome infusion. So I think the combination of the three is our essential philosophy because our clients have done extremely well. Again, we talked about data backed science and not only are we collecting pre and post labs, but we are also looking at epigenetic changes.
42:17
Dr. John Layke
And so stay tuned because we are working on clinical studies now.
42:25
Dafne Canales
And related to that question, we have a question about how long does each procedure take?
42:32
Dr. John Layke
So therapeutic plasma exchange usually takes if there are two IVs, which is ideal. It all depends on your IV access, right? So if you have got good veins and we can access two IVs, it only takes about two hours. And again that cycles through an entire blood volume. If you can only get one good vein, then obviously it takes almost twice as long. So it could be three and a half, four hours. As far as NK cell therapy, again this what we do is we usually at the time of tpe, we pull the blood for NK cells and then send it off. And so the re infusion of that NK cell can take 30 minutes. As far as the stem cell and exosome treatments, again same thing.
43:14
Dr. John Layke
We usually drip in that with a multi nutrient IV and something to help ramp up the immune system a little bit. And so it’ll take about an hour. And so these are all relatively short procedures. So even if you go down to Mexico and you’re planning on having a great time, you’re not going to ruin your entire trip.
43:33
Dafne Canales
We have another question here and it is do we need to do all the procedures in Mexico in Cabo?
43:41
Dr. John Layke
So eventually we will have all three of those in Mexico. The one we are allowed to do here in the United States is therapeutic plasma exchange. So we do that here in our Beverly Hills office. And you know, it’s a very comfortable procedure. You sit in a zero gravity massage chair, you watch Netflix or get, you know, get some work done and the machine itself is running in the background. But the, you know, as we talked about before, the code of federal regulations does not Allow us to do certain things here yet. And. And so that’s why the NK cell therapy and the IV infusions of stem cells and exosomes will be done in Mexico currently.
44:23
Dafne Canales
Thank you. And we have a question asking, do you feel anything during tpe? And it would be great for you to answer. Do you feel anything with stem cells or natural killer?
44:33
Dr. John Layke
Yes. So everyone’s a little different. I think immediately after the procedure, people feel invigorated, degraded. So during tpe, what we try to do is, you know, because when you do go through this filter, sometimes it leaches out calcium. And so were constantly monitoring you to make sure there’s no calcium depletion. And sometimes you can get little tingling of your fingers or things like that, but usually it’s a relatively innocuous process, meaning no one really feels anything. You relax, some people take naps, and afterwards they feel very invigorated. As far as, you know, I want to prepare you for any type of therapy like this. No one comes out feeling superhuman. That’s not the idea behind this. Although, you know, and again, having undergone the procedures myself, I noticed less aches and pains, better sleep, a little more energy.
45:32
Dr. John Layke
But really where I saw it were some of my inflammatory biomarkers and as well as even my inflammatory cholesterol. So that part was starting to climb up a little bit. And after a single session of therapeutic plasma exchange afterwards, it halved the number. And so to me, that’s a significant response. So when you get these therapies, especially the NK cell, so anytime you ramp up your immune system a little bit, sometimes you can, you know, get transient fevers, chills, or, you know, sometimes we get hot flashes, things like that, but sometimes even flu like symptoms for a short period of time, you know, maybe for a few hours, and then after that you’re completely fine. And most people, again, they have that invigorated feeling.
46:16
Dafne Canales
We have another question that was submitted in advance, and it talks about the 3D process and do we have to do the steps in a specific order or, you know, what do you recommend?
46:29
Dr. John Layke
That’s a great question. You do not. You know, the idea is we want to work around your schedule. And remember, there are three different categories when we look at aging. And so just because you do one first, it doesn’t preclude the other two from, you know, being just as effective. Right. And so even if you were to go to Mexico first and you started off with stem cell therapy and you came back here and did therapeutic plasma exchange or you know, we’re just trying to make it as efficient for you as possible. And so we tend to start with therapeutic plasma exchange because at the same time, you can pull your blood for natural killer cell therapy without an additional stick. And so, you know, but you can honestly do any one of these in any order, and you’ll have the same effect.
47:15
Dafne Canales
Thank you. And another question. Do you have testimonials from patients who have undergone these procedures?
47:21
Dr. John Layke
We do. And, you know, again, just like. Just like some of the other clinics, you. We’ve got our own celebs and sports figures and things like that, but we have regular everyday people who are just looking to slow down the signs of aging. And so, you know, happy to connect anyone with them so you can. They can tell you their experience.
47:42
Dafne Canales
Thank you, Dr. Layke. I’m gonna stop the questions there because we’re almost set time. And as a thank you to everyone for joining us today and staying until the end, we’re excited to announce the winner of our complimentary microneedling facial. It’s a transformative experience designed to rejuvenate the skin from the inside and out. And you can rewind the webinar to find out more about it because Dr. Layke spoke about that. We randomly selected one lucky attendee, and today’s winner is Carol Abney. So congratulations, Carol. If you want to just say hi in the chat and then you can contact us through the forums on the website. And if you didn’t win today, everyone, don’t worry. We’ll be offering more exclusive giveaways and special offers at future events. Please stay connected to see what’s coming next. I see Carol is saying woohoo. Doctor.
48:33
Dr. John Layke
All right, congrats. Congrats, Carol.
48:36
Dafne Canales
Congrats. Thank you, everyone, for joining us.